Skip to Content
Merck
  • Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography.

Preparation and chromatographic evaluation of a newly designed steviol glycoside modified-silica stationary phase in hydrophilic interaction liquid chromatography and reversed phase liquid chromatography.

Journal of chromatography. A (2015-03-03)
Tu Liang, Qing Fu, Aijin Shen, Hui Wang, Yu Jin, Huaxia Xin, Yanxiong Ke, Zhimou Guo, Xinmiao Liang
ABSTRACT

A diterpene glycoside compound, rebaudioside A (commonly abbreviated as RA), was immobilized onto porous silica surface through "thiol-ene" click chemistry strategy. The successful immobilization of the RA on the silica support was confirmed by FT-IR and elemental analysis. Chromatographic characteristics of the new stationary phase, named Click TE-RA, were evaluated by a set of diverse analytes such as carbohydrates, nucleosides, and organic acids in hydrophilic interaction liquid chromatography (HILIC) mode. The effects of water content, buffer pH and concentration were investigated and a typical HILIC retention feature of Click TE-RA was observed at high organic modifier content. The Click TE-RA stationary phase was further studied by a series of glycoside compounds. Tunable retention mechanisms from hydrophilic to hydrophobic interactions were observed. Separation of very polar compounds including oligosaccharides, nucleic acid bases and nucleosides using Click TE-RA in HILIC mode was successfully accomplished. In addition, separation of saponins both in HILIC and reversed-phase liquid chromatography (RPLC) modes was performed, demonstrating the presence of orthogonality between two different modes on Click TE-RA column. The multiple interactions induced by polar sugar group and hydrophobic aglycone group allowed this Click TE-RA to serve as a multi-mode stationary phase in two-dimensional liquid chromatography.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sucrose, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Supelco
Adenosine, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
USP
Sucrose, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Formic acid, ≥95%, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Supelco
Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
USP
Residual Solvent Class 2 - Acetonitrile, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
USP
Methyl alcohol, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Adenosine, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, for molecular biology, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sucrose, analytical standard, for enzymatic assay kit SCA20
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, ≥99.5% (GC), BioXtra
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, ≥99.5% (GC), Grade II, suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
β-D-Allose, rare aldohexose sugar
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, Grade I, ≥99% (GC), suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, ACS reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Adenosine
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
SKU
Pack Size
Availability
Price
Quantity
Supelco
Acetonitrile, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Millipore
Sucrose, suitable for microbiology, ACS reagent, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sucrose, meets USP testing specifications
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Uracil, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Adenosine, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Guanosine, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity