Skip to Content
Merck
  • Effects of glucose on cell viability and antioxidant and anti-inflammatory properties of phytochemicals and phytochemically modified membranes.

Effects of glucose on cell viability and antioxidant and anti-inflammatory properties of phytochemicals and phytochemically modified membranes.

The journal of physical chemistry. B (2014-09-24)
Teng Chang, Chandrasekaran Neelakandan, Linda DeFine, Thomas Alexander, Thein Kyu
ABSTRACT

By virtue of antioxidant and anti-inflammable properties, plant-derived phytochemicals such as mangiferin and genistein have attracted considerable attention for functionalization of polymeric hemodialysis (HD) membranes via solution blending. In-vitro dihydrorhodamine (DHR) assay of the genistein-modified membranes revealed drastic reduction in the level of the reactive oxygen species (ROS). In contrast, mangiferin-modified HD membrane manifested the pro-oxidant activity. We suspected that such difference in ROS generation may be attributed to the glucose unit on the xanthone backbone of mangiferin. This hypothesis was confirmed by comparing the ROS levels of genistein versus genistin, and mangiferin versus xanthone and 3,4,5,6-tetrahydroxyxanthone. Phytochemicals without the glucose unit show better antioxidant property related to the glycosides. Anti-inflammatory property was further conducted by measuring the level of TNF-α in blood after contacting with the same selected phytochemicals. Of particular interest is that the glucose unit promotes the generation of TNF-α.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
7-Aminoactinomycin D, ~97% (HPLC), powder
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dihydrorhodamine 123, ≥95%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Xanthone, 97%
SKU
Pack Size
Availability
Price
Quantity
USP
Xanthone, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
SKU
Pack Size
Availability
Price
Quantity
Supelco
Dimethyl sulfoxide, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ACS reagent, ≥99.9%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water
SKU
Pack Size
Availability
Price
Quantity
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
USP
Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
SKU
Pack Size
Availability
Price
Quantity