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Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Nickel, powder, <150 μm, 99.99% trace metals basis

Sigma-Aldrich

Nickel, powder, <1 μm, 99.8% trace metals basis

Sigma-Aldrich

Nickel, foil, thickness 0.25 mm, 99.995% trace metals basis

Nickel, foil, light tested, 50x50mm, thickness 0.0075mm, 99.95%

Nickel, foil, 0.5m coil, thickness 0.01mm, 99.95%

Nickel, tube, 500mm, outside diameter 5.9mm, inside diameter 5.65mm, wall thickness 0.125mm, hard, 99.5%

Nickel, foil, 10mm disks, thickness 0.008mm, 99.95%

Nickel, wire reel, 1m, diameter 0.75mm, as drawn, 99.99+%

Nickel, wire reel, 10m, diameter 0.05mm, as drawn, 99%

Nickel, foil, 4mm disks, thickness 0.05mm, 99.9%

Nickel, wire reel, 10m, diameter 0.25mm, as drawn, 99%

Nickel, foil, 4mm disks, thickness 0.05mm, as rolled, 99.99+%

Nickel, wire reel, 100m, diameter 0.25mm, as drawn, 99%

Nickel, foil, 4mm disks, thickness 0.008mm, 99.95%

Nickel, wire reel, 0.2m, diameter 0.4mm, as drawn, 99.99+%

Nickel, foil, 2m coil, thickness 0.1mm, annealed, 99%

Nickel, foil, 10mm disks, thickness 0.0025mm, 99.95%

Nickel, wire reel, 10m, diameter 0.5mm, hard, 99.98%

Nickel, foil, 4mm disks, thickness 0.015mm, 99.9%

Nickel, foil, 0.5m coil, thickness 0.015mm, 99.9%

Nickel, wire reel, 100m, diameter 0.5mm, hard, 99.98%

Nickel, foil, 300x300mm, thickness 2.0mm, annealed, 99%

Nickel, wire reel, 1m, diameter 0.05mm, as drawn, 99.99+%

Nickel, foil, 0.5m coil, thickness 0.30mm, as rolled, 99.98%

Nickel, wire reel, 10m, diameter 0.25mm, as drawn, 99.99+%

Nickel, foil, 0.5m coil, thickness 0.02mm, 99.9%

Nickel, foil, 4mm disks, thickness 0.025mm, as rolled, 99.99+%

Nickel, wire reel, 1000m, diameter 0.125mm, as drawn, 99%

Nickel, foil, 10mm disks, thickness 0.009mm, 99.95%

Nickel, wire reel, 1m, diameter 1.0mm, as drawn, 99.99+%