- A fluorescence "turn-on" chemodosimeter for Cu2+ in aqueous solution based on the ion promoted oxidation.
A fluorescence "turn-on" chemodosimeter for Cu2+ in aqueous solution based on the ion promoted oxidation.
Dalton transactions (Cambridge, England : 2003) (2012-02-24)
Junbo Li, Yang Zeng, Qihui Hu, Xianglin Yu, Jia Guo, Zhiquan Pan
PMID22358460
ABSTRACT
We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM, pH = 7.4) buffer containing 50% (v/v) water-CH(3)CN solution.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered