- Photocontrol of Endogenous Glycine Receptors In Vivo.
Photocontrol of Endogenous Glycine Receptors In Vivo.
Cell chemical biology (2020-08-28)
Alexandre M J Gomila, Karin Rustler, Galyna Maleeva, Alba Nin-Hill, Daniel Wutz, Antoni Bautista-Barrufet, Xavier Rovira, Miquel Bosch, Elvira Mukhametova, Elena Petukhova, Daria Ponomareva, Marat Mukhamedyarov, Franck Peiretti, Mercedes Alfonso-Prieto, Carme Rovira, Burkhard König, Piotr Bregestovski, Pau Gorostiza
PMID32846115
ABSTRACT
Glycine receptors (GlyRs) are indispensable for maintaining excitatory/inhibitory balance in neuronal circuits that control reflexes and rhythmic motor behaviors. Here we have developed Glyght, a GlyR ligand controlled with light. It is selective over other Cys-loop receptors, is active in vivo, and displays an allosteric mechanism of action. The photomanipulation of glycinergic neurotransmission opens new avenues to understanding inhibitory circuits in intact animals and to developing drug-based phototherapies.
MATERIALS