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796-0150

HPLC Analysis of Acidic Herbicides in Water on a Polymeric C18 Column after SPE using Supelclean™ ENVI™-Carb

application for SPE, application for HPLC

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technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: Fresh 1 L water samples, dechlorinated with sodium thiosulfate when necessary, at ambient temperature and pH.
SPE tube/cartridge: Supelclean ENVI-Carb, 250 mg/6 mL (57092)
condition: 10 mL DI water at 20 mL/min
sample addition: 0.9 L water sample at 20 mL/min
drying: 10 min using clean nitrogen
washing: 10 mL DI water at 20 mL/min
elution: 10 mL 0.1% phosphoric acid in methylene chloride:acetonitrile (80:20) at 5 mL/min
column: polymeric-coated silica-based PAH specialty column, 20 cm × 3 mm I.D., 5 μm (Supelco equivalent, SUPELCOSIL LC-PAH, available upon request)
mobile phase: gradient, [A]: 0.05% phosphoric acid in DI water; [B]: acetonitrile
flow rate: 0.5 mL/min
column temp.: 50 °C
detector: photodiode array- peak width: 0.053 min, sampling interval: 0.320 sec, monitor 210 nm & 225 nm
injection: 10 μL of extract (4-5 ppb each analyte in water)

suitability

application for HPLC
application for SPE

application(s)

environmental

Application

using Zymark AutoTrace Extraction WorkStation 1.20

Legal Information

ENVI is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description
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    2,4-D, analytical standard
    Picloram, BioReagent, suitable for plant cell culture
    2,4,5-T, PESTANAL®
    Pentachlorophenol
    Tetrachloroterephthalic acid
    Dicamba

analytical column

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