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SRP8029

Sigma-Aldrich

Flagellin from Salmonella typhimurium

>95% (SDS-PAGE)

Synonym(s):

FliC, Intelectin-1

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5 G
CHF 243.00

CHF 243.00

Estimated to ship on12 May 2025

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5 G
CHF 243.00

About This Item

CAS Number:
12777-81-0
UNSPSC Code:
12352202
NACRES:
NA.32

CHF 243.00

Estimated to ship on12 May 2025

Request a Bulk Order

biological source

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Assay

>95% (SDS-PAGE)

form

lyophilized

mol wt

~50 kDa by SDS-PAGE

packaging

pkg of 10 μg

impurities

<0.01 EU/μg endotoxin, tested

color

white

UniProt accession no.

P06179

shipped in

wet ice

storage temp.

−20°C

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M9163M7528M5017
Sigma-Aldrich

M0650

Medium 199

Sigma-Aldrich

M9163

Medium 199

Medium 199 HEPES Modification, with Earle&#8242;s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

M7528

Medium 199

Medium 199 With Earle&#8242;s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

M5017

Medium 199

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

components

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

L-glutamine: yes
phenol red: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

FliC (flagellin) is the subunit protein which polymerizes to form the filaments of bacterial flagella. The protein has four-domains (D0, D1, D2, D3).[1]
The encoded flagellin filament contains 55 kDa monomers.[2]

Biochem/physiol Actions

FliC (flagellin) can activate the innate immune system by TLR5 (toll like receptor 5) and is an important proinflammatory molecule.[1][3] FliC also induces the production of antibodies and is identified as an antigen by CD4+ T cells.[4]
It also stimulates the production of proinflammatory mediators : nitric oxide, interleukin 1 (IL-1), IL-6, IL-8, and tumor necrosis factor α.[5]

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    P27921901
    P21931204
    P26571701
    P1518706

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    Laís Rossi Perazza et al.
    The Journal of nutrition, 150(10), 2673-2686 (2020-09-05)
    Recent meta-analyses suggest that the consumption of fermented dairy products reduces type 2 diabetes and cardiovascular disease (CVD) risk, although the underlying mechanisms remain unclear. We evaluated whether dairy protein products modulated gut microbiota and cardiometabolic features in mouse models
    Mucosal administration of flagellin induces innate immunity in the mouse lung.
    Honko A N and Mizel S B
    Infection and Immunity, 72(11), 6676-6679 (2004)
    FliC-specific CD4+ T cell responses are restricted by bacterial regulation of antigen expression.
    Cummings LA
    Journal of Immunology, 174, 7929-7938 (2005)
    In vivo, fliC expression by Salmonella enterica serovar Typhimurium is heterogeneous, regulated by ClpX, and anatomically restricted.
    Cummings LA
    Molecular Microbiology, 61, 795-809 (2006)
    Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways.
    Zeng H, et al.
    American Journal of Physiology: Gastrointestinal and Liver Physiology, 290(1), G96-G108 (2006)
    View All Related Papers

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