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CWDS-MFB570

Sigma-Aldrich

ColorWheel® Antibody-Ready mFluor Blue 570 Dye

greener alternative

Synonym(s):

mFluor Blue 570

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About This Item

UNSPSC Code:
12352203

antibody form

purified antibody

Quality Level

antibody product type

primary antibodies

product line

ColorWheel®

form

lyophilized

purified by

gel-filtration chromatography

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

compatibility

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

greener alternative category

storage temp.

2-8°C

target post-translational modification

unmodified

General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

ColorWheel® Antibody-Ready mFluor Blue 570 Dye is designed for use with ColorWheel® Dye-Ready antibodies in Flow cytometry.

Application

Flow Cytometry: Excitation: 505 nm, Emission: 564 nm

Compatibility

Requires ColorWheel® Antibody(Sold Separately)

Target description

mFluor Blue 570 dye is an excellent alternative to R-Phycoerythrin (RPE) and exhibits similar spectral properties. It is a water-soluble dye and protein conjugates prepared using mFluor Blue 570 are well excited at 488 nm to give red fluorescence. Compared to RPE dyes, it offers greater photostability and is highly suitable for fluorescence imaging applications. mFluor Blue 570 exhibits an excitation max at 505 nm and an emission max at 564 nm. It displays an extinction coefficient of 12,000 cm-1 M-1.

Physical form

CWDS-MFB570-25UL Set Components

CWD-MFB570-25UL - ColorWheel® Antibody-Ready mFluor Blue 570 Dye

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL - ColorWheel® Finishing Buffer

Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
mFluor is a trademark of AAT Bioquest

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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