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C7104

Sigma-Aldrich

Anti-Cdc27 antibody,Mouse monoclonal

clone AF3.1, purified from hybridoma cell culture

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About This Item

MDL number:
MFCD01633250
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AF3.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 97 kDa

species reactivity

mouse, Xenopus, rat, bovine, canine, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using a HeLa Cell nuclear extract

isotype

IgG2b

UniProt accession no.

P30260

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDC27(996)
mouse ... Cdc27(217232)
rat ... Cdc27(360643)

General description

Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.
The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Application

Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
  • Immunocytochemistry using 4% paraformaldehyde fixation and Triton X-100 permeabilization
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections)
  • Immunoprecipitation.
  • Indirect ELISA
  • Microarray
  • Western blotting / immunoblotting at a concentration of 1-2μg/mL using a HeLa Cell nuclear extract.
  • Anaphase-promoting complex/cyclosome APC/C Purification.

Biochem/physiol Actions

CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.
The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Hui Guo et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), 5299-5304 (2015-02-15)
Cdc27, as a core component of anaphase-promoting complex (APC), is a cell cycle regulator, which participates in control of mitotic checkpoint and surveys the mitotic spindle to maintain chromosomal integrity. It was hypothesized that polymorphisms in cdc27 gene might contribute
A 20S complex containing CDC27 and CDC16 catalyzes the mitosis-specific conjugation of ubiquitin to cyclin B
King R W, e al.
Cell, 81(2), 279-288 (1995)
Liyong Zhang et al.
The Journal of biological chemistry, 286(12), 10041-10050 (2011-01-07)
Loss of TGF-β-induced growth inhibition is a hallmark of many human tumors. Previous studies implied that activation of the anaphase-promoting complex (APC/cyclosome) is involved in the TGF-β signaling pathway, which facilitates the destruction of SnoN, a transcriptional co-suppressor, which leads
Namit Singh et al.
The Journal of biological chemistry, 287(4), 2854-2862 (2011-12-06)
Microcephalin (MCPH1), the first gene identified as causative for primary recessive autosomal microcephaly, is aberrantly expressed in autism-like disorders and human malignancy of breast and ovarian origin. MCPH1, the encoded protein product, has been implicated in various cellular processes including
Sanjay Shete et al.
Cancer research, 71(24), 7568-7575 (2011-11-01)
Gliomas, which generally have a poor prognosis, are the most common primary malignant brain tumors in adults. Recent genome-wide association studies have shown that inherited susceptibility plays a role in the development of glioma. Although first-degree relatives of patients exhibit
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