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B4554

Sigma-Aldrich

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine

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About This Item

Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
10191-18-1
Molecular Weight:
213.25
Beilstein:
1781572
EC Number:
233-465-5
MDL number:
MFCD00007533
UNSPSC Code:
12161700
PubChem Substance ID:
24891788
NACRES:
NA.25

grade

BioPerformance Certified

Assay

≥99.0%

form

crystalline

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and bioburden, tested

pH

2.5-5.0 (25 °C, 213.3 g/L)

useful pH range

6.4-7.8

pKa (25 °C)

7.1

solubility

water: 0.5 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

storage temp.

room temp

SMILES string

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

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General description

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Application

BES buffer has been used:

  • as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
  • as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
  • as a component of diluent one for semen collection and cryopreservation

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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