Y0875

Select Yeast Extract

for use in microbial growth medium

• CAS Number: 8013-01-2
• MDL number: MFCD00132599
• UNSPSC Code: 41106212
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• form: powder
• technique(s): microbiological culture: suitable
• pH: 6.5-7.5(10% solution)
• solubility: H₂O: 100 mg/mL, clear to slightly hazy, yellow to brownish-yellow
• application(s): microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Yeast extract is the water-soluble portion of autolyzed yeast, often used in culture media. Yeast extract contains a mixture of amino acids, peptides, water soluble vitamins and carbohydrates.
Yeast extract is used in all solid media, on which stock cultures are maintained, due to its ability in promoting growth of most bacterial contaminants, thereby making them visible to identify. Yeast extract does not enhance the growth of wild-type cells but essential for auxotrophic mutants.[1]

Application

For general bacteriological use with a variety of microorganisms.

Select Yeast Extract has been used:

• as a supplement in the minimal sporulating medium (MSL) for the secretory production of human tumor necrosis factor-α (TNFα) using the carboxypeptidase Y (Cpy) signal peptide.[2]
• in the isolation of halophilic archaea.[3]
• as a component of microbial growth medium for cultivation of marine bacterial strain Zobellia uliginosa.[4]
  
  Suitable for use as nutritional source in microbial media.

Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

Other Notes

Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves