HCD8MAG-15K

MILLIPLEX^® Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

• Synonym(s): Human CD8 T cell cytokine panel, Human cytokine multiplex kit, Luminex^® human cytokine immunoassay, Millipore human cytokine panel
• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: standard curve range: 0.4-1,500 pg/mL; (IL-6); standard curve range: 0.5-2,000 pg/mL; (TNFα); standard curve range: 1-3,500 pg/mL; (MIP-1α); standard curve range: 1-5,000 pg/mL; (Granzyme B); standard curve range: 1-5,000 pg/mL; (IFNγ); standard curve range: 1-5,000 pg/mL; (IL-5); standard curve range: 10-50,000 pg/mL; (Perforin); standard curve range: 10-50,000 pg/mL; (sFasL); standard curve range: 2-10,000 pg/mL; (IL-4); standard curve range: 2-10,000 pg/mL; (sCD137); standard curve range: 2-7,500 pg/mL; (IL-13); standard curve range: 2-7,500 pg/mL; (IL-2); standard curve range: 20-100,000 pg/mL; (Granzyme A); standard curve range: 4-15,000 pg/mL; (GM-CSF); standard curve range: 400-1,650,000 pg/mL; (sFas); standard curve range: 5-20,000 pg/mL; (IL-10); standard curve range: 7-30,000 pg/mL; (MIP-1β)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

• Analytes: GM-CSF, sCD137, IFNγ, sFas, sFasL, Granzyme A, Granzyme B, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1α, MIP-1β, TNF-α, Perforin
• Recommended Sample type: serum, plasma or tissue/cell lysate and culture supernatant
• Recommended Sample dilution: Neat
• Assay Run Time: Overnight
• Research Category Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332 - H317 - H318 - H373 - H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3